Glycan array analysis of influenza H1N1 binding and release

• Type of Material: Article
• Authors: Shelly Gulati, University of Oklahoma; Yi Lasanajak, Emory University; David Smith, Emory University; Richard Cummings, Emory University; Gillian M. Air, University of Oklahoma
• Language: English
• Date: 2014-01-01
• Publisher: IOS Press
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © 2014 - IOS Press and the authors.
• Final Published Version (URL): http://dx.doi.org/10.3233/CBM-130376
• Title of Journal or Parent Work: Cancer Biomarkers
• ISSN: 1574-0153
• Volume: 14
• Issue: 1
• Start Page: 43
• End Page: 53
• Grant/Funding Information: This work was supported in part by grant AI 50933 from the National Institute of Allergy and Infectious Diseases.; The glycan array experiments were supported by grant GM62116 from the National Institute of General Medical Sciences to the Consortium for Functional Glycomics.
• Keywords: neuraminidase; Life Sciences & Biomedicine; REPLICATION; Oncology; hemagglutinin; MICROARRAY; SUBSTRATE-SPECIFICITY; Consortium for Functional Glycomics; Influenza virus; A VIRUS NEURAMINIDASE; Science & Technology; HEMAGGLUTININ-NEURAMINIDASE; Glycan array; SURFACE; FUNCTIONAL BALANCE; RECEPTOR SPECIFICITY
• Research Categories: Chemistry, Biochemistry; Biology, Virology

Abstract

Influenza viruses initiate infection by attaching to sialic acid receptors on the surface of host cells. It has been recognized for some time that avian influenza viruses usually bind to terminal sialic acid that is linked in the α2-3 configuration to the next sugar while human viruses show preference for α2-6 linked sialic acid. With developments in synthetic chemistry and chemo-enzymatic methods of synthesizing quite complex glycans, it has become clear that the binding specificity extends beyond the sialic acid, and this has led to considerable interest in developing glycan reagents that could be used either as a diagnostic tool for particular influenza viruses, or to identify cells that are susceptible to infection by certain influenza viruses. Here we describe the use of the Consortium for Functional Glycomics Glycan Array to investigate binding specificity of influenza hemagglutinin and cleavage by neuraminidase, using seasonal and pandemic H1N1 influenza viruses as examples, and compare the results with published data using other array methods.