MIRAGE: The minimum information required for a glycomics experiment

• Type of Material: Article
• Authors: William S. York, University of Georgia; Sanjay Agravat, Emory University; Kiyoko F. Aoki-Kinoshita, Soka University; Ryan McBride, Scripps Research Institute; Matthew P. Campbell, Macquarie University; Catherine E. Costello, Boston University; Anne Dell, Imperial College London; Ten Feizi, Imperial College London; Stuart M. Haslam, Imperial College London; Niclas Karlsson, University of Gothenburg; Kay-Hooi Khoo, Acad Sinica; Daniel Kolarich, Max Planck Institute of Colloids and Interfaces; Yan Liu, Imperial College London; Milos Novotny, Indiana University; Nicolle H. Packer, Macquarie University; James C. Paulson, Scripps Research Institute; Erdmann Rapp, Max Planck Institute for Dynamics of Complex Technical Systems; Rene Ranzinger, University of Georgia; Pauline M. Rudd, NIBRT – National Institute for Bioprocessing Research and Training; David Smith, Emory University; Weston B. Struwe, University of Oxford; Michael Tiemeyer, University of Georgia; Lance Wells, University of Georgia; Joseph Zaia, Macquarie University; Carsten Kettner, Beilstein-Institut
• Language: English
• Date: 2014-05-01
• Publisher: Oxford University Press (OUP): Policy B - Oxford Open Option B
• Publication Version: Final Published Version
• Copyright Statement: © The Author 2014.
• License: Creative Commons Attribution-Noncommercial 3.0 Unported
• Final Published Version (URL): http://dx.doi.org/10.1093/glycob/cwu018
• Title of Journal or Parent Work: Glycobiology
• ISSN: 0959-6658
• Volume: 24
• Issue: 5
• Start Page: 402
• End Page: 406
• Grant/Funding Information: NK's contribution to this initiative is supported by The Swedish Foundation for International Cooperation in Research and Higher Education.; Both, ER and PMR have a grant from the European Union's Seventh Framework Programme (FP7-Health-F5-2011;, grant agreement no 278535 “HighGlycan”); in addition, PMR has a grant from the European Union's Seventh Framework Programme (FP7/2007-2013;, grant no. 259896); AD and SH are supported by the Biotechnology and Biological Sciences Research Council (grants BB/K016164/1; and BB/I017011/1); MC and NP are supported by the Australian Government project NeCTAR financed by the Education Investment Fund; JZ and CC are supported by the NIH/NIGMS grant P41GM104603; WY, RR, MT and LW are supported as part of the NIH/NIGMS funded National Center for Glycomics and Glycoproteomics (8P41GM103490).; Funding to pay the Open Access publication charges for this article was provided by Beilstein-Institut.; TF and YL are supported by a Wellcome Trust Biomedical Resource grant WT099197MA.; The Beilstein-Institut, a non-profit foundation established under civil law and located in Frankfurt am Main, Germany, has provided funding for advancement of the MIRAGE initiative.; This work has also been supported by several other funding agencies: DK and ER are both supported by the Max Planck Society, and in addition, DK is funded by the Marie Curie grant PCIG09-GA-2011-293847.
• Keywords: Science & Technology; BIOCHEMISTRY & MOLECULAR BIOLOGY; Life Sciences & Biomedicine; Biochemistry & Molecular Biology; PROJECT; PROTEOMICS; STANDARDS
• Research Categories: Chemistry, Biochemistry; Engineering, Biomedical

Abstract

The MIRAGE (minimum information required for a glycomics experiment) initiative was founded in Seattle, WA, in November 2011 in order to develop guidelines for reporting the qualitative and quantitative results obtained by diverse types of glycomics analyses, including the conditions and techniques that were applied to prepare the glycans for analysis and generate the primary data along with the tools and parameters that were used to process and annotate this data. These guidelines must address a broad range of issues, as glycomics data are inherently complex and are generated using diverse methods, including mass spectrometry (MS), chromatography, glycan array-binding assays, nuclear magnetic resonance (NMR) and other rapidly developing technologies. The acceptance of these guidelines by scientists conducting research on biological systems in which glycans have a significant role will facilitate the evaluation and reproduction of glycomics experiments and data that is reported in scientific journals and uploaded to glycomics databases. As a first step, MIRAGE guidelines for glycan analysis by MS have been recently published (Kolarich D, Rapp E, Struwe WB, Haslam SM, Zaia J., et al. 2013. The minimum information required for a glycomics experiment (MIRAGE) project-Improving the standards for reporting mass spectrometry-based glycoanalytic data. Mol. Cell Proteomics. 12:991-995), allowing them to be implemented and evaluated in the context of real-world glycobiology research. In this paper, we set out the historical context, organization structure and overarching objectives of the MIRAGE initiative.