Localization of Glucose Transporter 10 to Hair Cells' Cuticular Plate in the Mouse Inner Ear

• Type of Material: Article
• Authors: Bei Chen, Zhengzhou University; Yunfeng Wang, Fudan University; Manying Geng, Zhengzhou University; Xi Erick Lin, Emory University; Wenxue Tang, Zhengzhou University
• Language: English
• Date: 2018-01-01
• Publisher: Hindawi Publishing Corporation
• Publication Version: Final Published Version
• Copyright Statement: © 2018 Bei Chen et al.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1155/2018/7817453
• Title of Journal or Parent Work: BioMed Research International
• ISSN: 2314-6133
• Volume: 2018
• Start Page: 7817453
• End Page: 7817453
• Grant/Funding Information: This study was supported by grants to Xi Lin from the National Institute on Deafness and other Communication Disorders (RO1-DC04709 and RO1-DC006483), a grant to Wenxue Tang from the NIH (R21 DC008672), and a grant to Bei Chen from the Key Research Items of the Higher Education Institutions of Henan Province, China (17A310033).
• Keywords: Life Sciences & Biomedicine; Science & Technology; Medicine, Research & Experimental; OXIDATIVE STRESS; EXPRESSION; ARTERIAL TORTUOSITY SYNDROME; Biotechnology & Applied Microbiology; Research & Experimental Medicine; DIABETES-MELLITUS; GLUT FAMILY
• Research Categories: Health Sciences, General; Biology, Cell

Abstract

This study aimed to investigate the localization pattern of glucose transporters (Gluts) in mouse cochlea. Genome-wide gene expression analysis using CodeLink™ bioarrays indicated that Glut1 and Glut10 were highly expressed (10-fold) in mouse cochlea compared with the other members of glucose transporters (Glut2-6, Glut8, and Glut9). Semiquantitative RT-PCR and western blotting confirmed that Glut10 expression in mouse cochlea was high throughout the embryogenesis and postnatal development. Immunofluorescent staining showed that Glut10 protein was localized in the cuticular plate of the outer and inner cochlear hair cells and in the ampullary crest of the vestibular system. Based on these results, it was supposed that Glut10 may contribute to glucose transport from the endolymph to the hair cells across the cuticular plate.