Mitochondrial H2S Regulates BCAA Catabolism in Heart Failure

• Type of Material: Article
• Authors: Zhen Li, Louisiana State University; Huijing Xia, Louisiana State University; Thomas E Sharp, III, Louisiana State University; Kyle B LaPenna, Louisiana State University; John W Elrod, Temple University; Kevin M Casin, Emory University; Ken Liu, Emory University; John Calvert, Emory University; Vinh Q Chau, Virginia Commonwealth University; Fadi N Salloum, Virginia Commonwealth University; Shi Xu, Brown University; Ming Xian, Brown University; Noriyuki Nagahara, Nippon Medical School, Tokyo; Traci T Goodchild, Louisiana State University; David J Lefer, Louisiana State University
• Language: English
• Date: 2022-07-22
• Publisher: LIPPINCOTT WILLIAMS & WILKINS
• Publication Version: Author Accepted Manuscript (After Peer Review)
• Copyright Statement: © American Heart Association, Inc. All rights reserved.
• Final Published Version (URL): http://dx.doi.org/10.1161/CIRCRESAHA.121.319817
• Title of Journal or Parent Work: CIRCULATION RESEARCH
• Volume: 131
• Issue: 3
• Start Page: 222
• End Page: 235
• Grant/Funding Information: This work was supported by Grants from NIH National Heart, Lung, and Blood Institute (R01 HL146098, R01 HL146514, R01 HL137711) to DJL, (R01 HL151398) to DJL and MX, (R01 HL133167, R35 HL155651) to FNS, American Heart Association Postdoctoral Grant (20POST3520075) to ZL, and (18POST34020143) to TES.
• Keywords: amino acids; ENZYME; SULFANE SULFUR; heart failure; Peripheral Vascular Disease; cell death; 3-MERCAPTOPYRUVATE SULFURTRANSFERASE; Hematology; branched-chain; Life Sciences & Biomedicine; DISEASE; Science & Technology; HYDROGEN-SULFIDE; hydrogen sulfide; mitochondrial respiration; Cardiac & Cardiovascular Systems; Cardiovascular System & Cardiology; CHAIN AMINO-ACIDS
• Research Categories: Chemistry, Biochemistry; Biology, Physiology

Abstract

Background: Hydrogen sulfide (H2S) exerts mitochondria-specific actions that include the preservation of oxidative phosphorylation, biogenesis, and ATP synthesis, while inhibiting cell death. 3-MST (3-mercaptopyruvate sulfurtransferase) is a mitochondrial H2S-producing enzyme whose functions in the cardiovascular disease are not fully understood. In the current study, we investigated the effects of global 3-MST deficiency in the setting of pressure overload-induced heart failure. Methods: Human myocardial samples obtained from patients with heart failure undergoing cardiac surgeries were probed for 3-MST protein expression. 3-MST knockout mice and C57BL/6J wild-type mice were subjected to transverse aortic constriction to induce pressure overload heart failure with reduced ejection fraction. Cardiac structure and function, vascular reactivity, exercise performance, mitochondrial respiration, and ATP synthesis efficiency were assessed. In addition, untargeted metabolomics were utilized to identify key pathways altered by 3-MST deficiency. Results: Myocardial 3-MST was significantly reduced in patients with heart failure compared with nonfailing controls. 3-MST KO mice exhibited increased accumulation of branched-chain amino acids in the myocardium, which was associated with reduced mitochondrial respiration and ATP synthesis, exacerbated cardiac and vascular dysfunction, and worsened exercise performance following transverse aortic constriction. Restoring myocardial branched-chain amino acid catabolism with 3,6-dichlorobenzo1[b]thiophene-2-carboxylic acid (BT2) and administration of a potent H2S donor JK-1 ameliorates the detrimental effects of 3-MST deficiency in heart failure with reduced ejection fraction. Conclusions: Our data suggest that 3-MST derived mitochondrial H2S may play a regulatory role in branched-chain amino acid catabolism and mediate critical cardiovascular protection in heart failure.