Bioactive flavonoid p-hydroxycinnamic acid stimulates osteoblastogenesis and suppresses adipogenesis in bone marrow culture

• Type of Material: Article
• Authors: Masayoshi Yamaguchi, Emory University; Clifton A. Baile, University of Georgia; Shijun Zhu, Emory University; Mamoru Shoji, Emory University
• Language: English
• Date: 2013-12-01
• Publisher: Springer Verlag
• Publication Version: Final Published Version
• Copyright Statement: © The Author(s) 2013.This article is distributed under the terms of the Creative Commons Attribution License, which permits any use, distribution and reproduction in any medium, provided the original author(s) and the source are credited.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1007/s00441-013-1707-6
• Title of Journal or Parent Work: Cell and Tissue Research
• ISSN: 0302-766X
• Volume: 354
• Issue: 3
• Start Page: 743
• End Page: 750
• Grant/Funding Information: M.Y. was partly supported by an Award of the Mishima Kaiun Memorial Foundation and by a Senji Miyata Foundation Award (Japan).
• Keywords: MESENCHYMAL STEM-CELLS; RATS; p-Hydroxycinnamic acid; FEMORAL TISSUES; DIFFERENTIATION; Cell Biology; KAPPA-B ACTIVATION; Life Sciences & Biomedicine; 3T3-L1 ADIPOCYTES; Science & Technology; PHOSPHORYLATION; 3T3-L1 preadipocytes; Osteoblastogenesis; GENE-EXPRESSION; PPAR-GAMMA; Insulin; Adipogenesis; MINERALIZATION
• Research Categories: Biology, Microbiology; Biology, General

Abstract

The bioactive flavonoid p-hydroxycinnamic acid (HCA), which is an intermediate-metabolic substance in plants and fruits, is synthesized from tyrosine. The biological effect of HCA is poorly understood. Among cinnamic acid and its related compounds, HCA has a specific-anabolic effect on bone, being found to stimulate osteoblastogenesis and to inhibit osteoclastogenesis through the suppression of NF-κB signaling, thereby preventing bone loss. Bone marrow mesenchymal stem cells give rise to ostoblasts and adipocytes. HCA might therefore have effects on osteoblastogenesis and adipogenesis in bone marrow culture. This study demonstrates (1) that HCA has stimulatory effects on osteoblastogenesis and mineralization and suppressive effects on adipogenesis in mouse bone marrow culture and (2) that HCA depresses adipogenesis in mouse 3T3-L1 preadipocytes in vitro. Such effects of HCA might be involved in the differentiation of mesenchymal stem cells.