Plasma CXCL8 and MCP-1 as biomarkers of latent tuberculosis infection

• Type of Material: Article
• Authors: Marie Larsson, Linkoping University; Esaki M. Shankar, Central University of Tamil Nadu; Sivadoss Raju, State Public Health Laboratory; Sivaprakasam T. Selvavinayagam, State Public Health Laboratory; Bijulal Aswathy, Central University of Tamil Nadu; Yean K. Yong, Xiamen University; Asha Frederick, National Tuberculosis Elimination Programme; Lakshmi Murali, National Tuberculosis Elimination Programme; Vasudevan Kalaivani, State Public Health Laboratory; Karishma S. Jith, Central University of Tamil Nadu; Manivannan Rajeshkumar, State Public Health Laboratory; Adukkadukkam Anusree, Central University of Tamil Nadu; Meganathan Kannan, Central University of Tamil Nadu; Natarajan Gopalan, Central University of Tamil Nadu; Ramachandran Vignesh, Universiti Kuala Lumpur; Amudhan Murugesan, The Government Theni Medical College and Hospital; Hong Yien Tan, Xiamen University; Ying Zhang, Xiamen University; Samudi Chandramathi, University of Malaya; Munusamy Ponnan Sivasankaran, Seattle Children’s Research Institute; Sakthivel Govindaraj, Emory University; Siddappa N. Byrareddy, University of Nebraska; Vijayakumar Velu, Emory University
• Language: English
• Date: 2023-08-09
• Publisher: NIH
• Publication Version: Preprint (Prior to Peer Review)
• Copyright Statement: The copyright holder for this preprint is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
• License: Creative Commons Attribution 4.0 International
• Final Published Version (URL): https://doi.org/10.1101%2F2023.08.07.23293767
• Title of Journal or Parent Work: medRxiv
• Grant/Funding Information: EMS is funded by the Department of Science and Technology-Science and Engineering Research Board, Government of India (CRG/2019/006096). This work is also supported by grants through AI52731, the Swedish Research Council, the Swedish, Physicians against AIDS Research Foundation, the Swedish International Development Cooperation Agency, SIDA SARC, VINNMER for Vinnova, Linköping University Hospital Research Fund, CALF, and the Swedish Society of Medicine (to ML). H.Y.T. is supported by Xiamen University Research Funding (XMUMRF/2020-C5/ITCM/0003 and VV is supported by: The NIH Office of Research Infrastructure Programs (P51 OD011132 to ENPRC), and Emory CFAR (P30 AI050409).
• Keywords: latent infection; IFN-γ; LTBI; TB elimination; Cytokines; IGRA
• Research Categories: Biology, Virology; Health Sciences, Public Health

Abstract

Background Early detection of latent tuberculosis infection (LTBI) is critical to TB elimination in the current WHO vision of End Tuberculosis Strategy. Methods We investigated whether detecting plasma cytokines could aid in diagnosing LTBI across household contacts (HHCs) positive for IGRA, HHCs negative for IGRA, and healthy controls. We also measured the plasma cytokines using a commercial Bio-Plex Pro Human Cytokine 17-plex assay. Results Increased plasma CXCL8 and decreased MCP-1, TNF-α, and IFN-γ were associated with LTBI. Regression analysis showed that a combination of CXCL8 and MCP-1 increased the risk of LTBI among HHCs to 14-fold. Conclusions We postulated that CXCL8 and MCP-1 could be the surrogate biomarkers of LTBI, especially in resource-limited settings.