Molecular Diagnosis of Coronavirus Disease 2019.

• Type of Material: Article
• Authors: Claudia C. Dos Santos, University of Toronto; Barbara Zehnbauer, Emory University; Uriel Trahtemberg, Unity Health Toronto; John Marshall, University of Toronto
• Language: English
• Date: 2020-09
• Publisher: Wolters Kluwer Health, Inc
• Publication Version: Final Published Version
• Copyright Statement: © 2020 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of the Society of Critical Care Medicine
• License: Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International
• Final Published Version (URL): http://dx.doi.org/10.1097/CCE.0000000000000184
• Title of Journal or Parent Work: Crit Care Explor
• Volume: 2
• Issue: 9
• Start Page: e0184
• End Page: e0184
• Keywords: coronavirus disease 2019; coronavirus disease 2019 serologic tests; severe acute respiratory syndrome coronavirus 2; nucleic acid amplification tests; real-time reverse transcription polymerase chain reaction diagnostic primer panel
• Research Categories: Biology, Molecular; Health Sciences, Public Health

Abstract

Objectives: To review molecular diagnostics for coronavirus disease 2019. The world is in the midst of a coronavirus disease 2019 pandemic. Containing the spread of the severe acute respiratory distress coronavirus is critical. Instrumental to the future success is the ability to reliably and reproducibly detect this inciting pathogen to inform public health containment policies and treatment decisions. Data Sources: Molecular diagnostics focusing on molecular detection methodologies for detection of the virus and the presence of the disease. Study Selection: Narrative review. Data Extraction: Literature, PubMed, Scopus, and official government documents. Data Synthesis: Diagnosing severe acute respiratory syndrome coronavirus is done through real-time reverse transcriptase-polymerase chain reaction tests, cell culture, and serology. For patients, diagnostics are an integral part of a full medical history, physical examinations, blood tests, and diagnostic imaging. Conclusions: Here, we review current approaches to the molecular diagnosis of coronavirus disease 2019.