Publication

Insights into T cell recognition of antigen: significance of two-dimensional kinetic parameters

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Last modified
  • 02/20/2025
Type of Material
Authors
    Lindsay J. Edwards, Emory UniversityVeronika I. Zarnitsyn, Georgia Institute of Technology Jennifer D. Hood, Emory UniversityBrian Evavold, Emory UniversityCheng Zhu, Emory University
Language
  • English
Date
  • 2012-04-20
Publisher
  • Frontiers Research Foundation
Publication Version
Copyright Statement
  • © 2012 Edwards, Zarnitsyna, Hood, Evavold and Zhu.
License
Final Published Version (URL)
Title of Journal or Parent Work
ISSN
  • 1664-3224
Volume
  • 3
Issue
  • 86
Start Page
  • 1
End Page
  • 9
Grant/Funding Information
  • Support was provided by NIH (R01GM096187, R01NS062358, R01NS071518) and the National Multiple Sclerosis Society(RG4482).
Abstract
  • The T cell receptor (TCR) interacts with peptide-major histocompatibility complex (pMHC) to enable T cell development and trigger adaptive immune responses. For this reason, TCR:pMHC interactions have been intensely studied for over two decades. However, the details of how various binding parameters impact T cell activation remain elusive. Most measurements were made using recombinant proteins by surface plasmon resonance, a three-dimensional (3D) technique in which fluid-phase receptors and ligands are removed from their cellular environment. This approach found TCR:pMHC interactions with relatively low affinities and slow off-rates for agonist peptides. Newer generation techniques have analyzed TCR:pMHC interactions in two dimensions (2D), with both proteins anchored in apposing plasma membranes. These approaches reveal in situ TCR:pMHC interaction kinetics that are of high affinity and exhibit rapid on- and off-rates upon interaction with agonist ligands. Importantly, 2D binding parameters correlate better with T cell functional responses to a spectrum of ligands than 3D measures.
Author Notes
  • Correspondence: Brian D. Evavold, Department of Microbiology and Immunology, Emory University, 1510 Clifton Road, Atlanta, GA 30322, USA. e-mail: bevavol@emory.edu; Cheng Zhu, Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0363, USA. e-mail: cheng.zhu@bme.gatech.edu
Research Categories
  • Health Sciences, Immunology
  • Biology, Cell

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