Publication

Characterization of a Species-Specific Insulinase-Like Protease in Cryptosporidium parvum.

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Last modified
  • 05/15/2025
Type of Material
Authors
    Shijing Zhang, East China University of Science and TechnologyYuping Wang, East China University of Science and TechnologyHaizhen Wu, East China University of Science and TechnologyNa Li, South China Agricultural UniversityJianlin Jiang, Emory UniversityYaqiong Guo, South China Agricultural UniversityYaoyu Feng, East China University of Science and TechnologyLihua Xiao, South China Agricultural University
Language
  • English
Date
  • 2019
Publisher
  • Frontiers Media
Publication Version
Copyright Statement
  • © 2019 Zhang, Wang, Wu, Li, Jiang, Guo, Feng and Xiao.
License
Final Published Version (URL)
Title of Journal or Parent Work
ISSN
  • 1664-302X
Volume
  • 10
Start Page
  • 354
End Page
  • 354
Grant/Funding Information
  • This study was supported by the National Nature Science Foundation of China (31630078 and 31425025).
Abstract
  • Cryptosporidium parvum is an intracellular protozoan that can cause severe diarrhea in humans and various mammals. Results of a comparative genomic analysis indicated that genes encoding two C. parvum-specific insulinase-like proteases (INS19 and INS20), cgd6_5510 and cgd6_5520, are lost in many Cryptosporidium species. In this study, we provided evidence indicating that cgd6_5510 and cgd6_5520 are fragments of a full gene (cgd6_5520-5510) encoding one insulinase-like protease (INS20-19) that is similar in structure to classic insulinases. We expressed cgd6_5510 in Escherichia coli for antiserum preparation and found the protein (INS19) that was partially degraded. A ~180 kDa protein of INS20-19 was specifically recognized by the polyclonal anti-INS19 antiserum in sporozoite lysate. We observed that INS20-19 is likely a protein with high expression in the apical region of sporozoites, and neutralization of the protein led to a partial reduction of parasite load in HCT-8 and MDBK cell cultures at 24 h. Taken together, our findings support the involvement of INS20-19 in the invasion or early developmental process of C. parvum.
Author Notes
Keywords
Research Categories
  • Health Sciences, Immunology
  • Biology, Animal Physiology

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