Field Detection of Schistosoma japonicum Cercariae in Environmental Water Samples by Quantitative PCR

• Type of Material: Article
• Authors: Caitlin Worrell, Emory University; Ning Xiao, Sichuan Center for Disease Control and Prevention; Jorge Vidal Graniel, Emory University; Lin Chen, Sichuan Center for Disease Control and Prevention; Bo Zhong, Sichuan Center for Disease Control and Prevention; Justin V Remais, Emory University
• Language: English
• Date: 2011-03
• Publisher: American Society for Microbiology (ASM)
• Publication Version: Final Published Version
• Copyright Statement: © 2011, American Society for Microbiology
• Final Published Version (URL): http://aem.asm.org/content/77/6/2192
• Title of Journal or Parent Work: Applied and Environmental Microbiology
• Volume: 77
• Issue: 6
• Start Page: 2192
• End Page: 2195
• Grant/Funding Information: This work was supported in part by the International Foundation for Ethical Research and by the Ecology of Infectious Disease program of the National Science Foundation under grant no. 0622743.; Mouse bioassays were supported by the National Institute for Allergy and Infectious Diseases grant no. R01AI068854.; The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
• Research Categories: Biology, Microbiology

Abstract

A species-specific quantitative PCR (qPCR) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of S. japonicum in surface waters. The novel methods were capable of capturing cercariae and, with subsequent analysis through qPCR, detecting the presence of a minimum of 1 cercaria.