HUMAN T-LYMPHOCYTE SUB-POPULATIONS DEFINED BY FC-RECEPTORS AND MONOCLONAL-ANTIBODIES - COMPARISON

• Type of Material: Article
• Authors: E.L. Reinherz, Sidney Farber Cancer Institute; L. Moretta, University of Genova; M. Roper, University of Alabama at Birmingham; J.M. Breard, Sidney Farber Cancer Institute; M.C. Mingari, University of Genova; Max Cooper, Emory University; S.F. Schlossman, Sidney Farber Cancer Institute
• Language: English
• Date: 1980-01-01
• Publisher: Rockefeller University Press
• Publication Version: Final Published Version
• Copyright Statement: © Rockefeller University Press.
• Final Published Version (URL): http://dx.doi.org/10.1084/jem.151.4.969
• Title of Journal or Parent Work: Journal of Experimental Medicine
• ISSN: 0022-1007
• Volume: 151
• Issue: 4
• Start Page: 969
• End Page: 974
• Grant/Funding Information: Supported by grants AI 12069, CA 19589, CA 06516, and CA 16673 from the National Institutes of Health.
• Keywords: Science & Technology; Research & Experimental Medicine; Life Sciences & Biomedicine; Immunology
• Research Categories: Health Sciences, Immunology; Biology, Microbiology

Abstract

Human T cell subpopulations have been defined on the basis of differential expression of either Fc receptors or specfic cell-surface antigens. In this study, we utilized a series of monoclonal antibodies reactive with T cells, monocytes, and Ia antigens to characterize isolated subpopulations of T cells bearing receptors for the Fc portion of IgG (Tγ) and subpopulations of T cells bearing receptors for the Fc portion of IgM Tμ. The results showed that the Tμ population contained both inducer (OKT4 +) and cytotoxic/suppressor (OKT5 +) populations and was similar to the unfractionated T cell population, whereas the Tγ subset contained few T lymphocytes (OKT3 +) and was not enriched for either T cell subset defined by these monoclonal antibodies. Rather, the Tγ population was comprised largely of Ia - cells possessing a monocyte antigen (OKM1 +). In reciprocal studies, it was found that both isolated OKT4 + and OKT5 +T cell subsets contained few Tγ cells, whereas both subsets were mainly comprised of Tμ cells. We conclude that there is little correlation between T cell subsets defined by these monoclonal antibodies and those defined by Fc receptors.